Currently, we are interested in a protein named LGN, which is important for mammalian cellular division by playing a significant role in cell polarity and the alignment of the mitotic spindles.1-3 LGN is also found in high concentrations in certain cancer cells.4 Determining the crystal structure of LGN may lead to the development of pharmacological drugs to slow the onset or spread of these cancer cells. The immediate goal of the lab is to optimize the purification scheme to obtain 95% pure LGN using various types of chromatography. Once 95% purity is achieved, conditions to grow LGN crystals will be determined and analyzed to solve the three dimensional structure of LGN.
1. Ganderath, Stollerwerk, Grieg, Goodman, Kane and Klamph. (1999) Development, 126, 1781-1791.
2. Zheng, Zhu, Wan, Liu, Xiao, Siderovski and Du. (2010) J of Cell Bio, 189, 275-288.
3. Nipper, Siller, Smith, Doe and Prehoda. (2007) PNAS, 104, 14306-14311.
4. Fukukawa, Ueda, Nishidate, Katagiri and Nakamura. (2010) Genes, Chromosomes and Cancer, 49, 861-872.
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