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2012 Master's Level Graduate Research Conference

Session I - Session II - Session III - Session IV

Gastrointestinal Motility Patterns in the Zebrafish

Background: Gastrointestinal (GI) motility is the spontaneous, rhythmic, coordinated contraction of smooth muscle. The function of GI motility is to mix intestinal contents for nutrient absorption and propulsion of luminal contents to eliminates waste and minimizes bacterial overgrowth. The zebrafish GI tract is anatomically similar to humans and since larvae are transparent GI motility can be observed in intact animals. Several research laboratories measure GI motility but standard protocols have not been developed. Testing compounds with well characterized effects on mouse GI motility is necessary for validation of the zebrafish model. Aim: Examine zebrafish GI motility during the fed and fasted states. Acute exposure to the drugs cisapride, niflumic acid, and DIDS will also be characterized in the zebrafish model system. Methods: GI motility was measured in 7 dpf larvae using time lapse imaging and subsequent analysis of digitized images. Larvae were anesthetized, immobilized in agar, and positioned for image collection using an inverted microscope. Images were acquired every second for 10 minutes and analyzed using custom written software routines. Results: GI motility was not different in fed compared to fasted larvae. Cisapride, niflumic acid and DIDS disrupted GI motility patterns in the zebrafish. Conclusions: These data suggest that feeding has little influence on zebrafish GI motility patterns, thus fed and fasted protocols are comparable. Compounds that influence GI motility in humans and mice have similar effects on the zebrafish, contributing to validation of the zebrafish model system for human GI motility research. Supported by NIH DK07158802

Presenter: Amanda Diamond (The College at Brockport) --
Topic: Biology - Panel
Location: 215 Hartwell
Time: 10 am (Session I)