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2012 Master's Level Graduate Research Conference

Session I - Session II - Session III - Session IV

Characterization of Anoctamin 1 in Zebrafish Gastrointestinal Motility

Anoctamin 1 (ano1) codes for a calcium activated chloride channel. Calcium activated chloride channels have recently been shown to be expressed on interstitial cells of Cajal (ICC) in humans and mice. ICC function as pacemaker cells in gastrointestinal (GI) tract motility. Ano1 is a selective marker for ICC and inhibiting ano1 disrupts coordinated motility patterns in the GI tract. The primary goal of this project is to determine the functional role of ano1 in the zebrafish GI tract. Morpholino antisense oligonucleotides (MO) will be used to reduce, or knock down, the expression of ano1 in zebrafish. The effects of MO knockdown lessen with develop and therefore MO efficacy will be measured using polymerase chain reaction (PCR) at several time points during development. GI motility will be measured at 7 days post fertilization using digital imaging of MO injected larvae and compared to controls. Preliminary designs of MO sequences have been examined for off-target interactions that could reduce ano1 knockdown effects, or that could knockdown other unrelated genes. We anticipate that MO knockdown of ano1 will result in uncoordinated GI motility patterns in zebrafish. MO knockdown of ano1 will contribute to a better understanding of the role of ICC in GI motility, and will further develop the zebrafish model system. Supported by NIH DK071588-02

Presenter: Cyrus Salehi (The College at Brockport) -- csale1@brockport.edu
Topic: Biology - Panel
Location: 215 Hartwell
Time: 9:20 am (Session I)