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2012 Master's Level Graduate Research Conference

Session I - Session II - Session III - Session IV

Spatial Expression Profile of Anoctamin 1 in the Zebrafish Gastrointestinal Tract

Anoctamin-1 (Ano1) is a Ca2+-activated Cl- channel functionally important in many diverse tissues, supporting olfactory and photoreceptor signaling, fluid secretion, and gastrointestinal (GI) smooth muscle pacemaking. Previous studies showed that Ano1 is expressed in interstitial cells of Cajal (ICC) and is necessary for normal GI motility patterns in both mouse and human tissue. We have shown that the gene for Ano1 is expressed on a network of cells in the GI tract using both anti-Ano1 antibodies and polymerase chain reaction (PCR). The objective for this project is to confirm spatial expression patterns for the gene ano1 at the transcriptional level. The current formatting conventions dictate printed gene symbols for zebrafish protein (Ano1) and gene (ano1). In situ hybridization (ISH) detects the location of messenger RNA (mRNA) in fixed tissues using a sequence specific for a target gene making it a gene-specific probe. A probe against ano1 will be constructed and used on transverse sections of fixed adult zebrafish GI tissue to determine spatial expression patterns. Since the presence of Ano1 has been confirmed in mice and humans, we anticipate ano1 will be expressed in zebrafish ICC. Confirmation of ano1 expression in zebrafish GI tissue is the first step towards understanding its functional role in motility, and will contribute to understanding its role in human GI motility. Supported by NIH DK07158801

Presenter: Steven Lane (The College at Brockport) -- slane1@brockport.edu
Topic: Biology - Panel
Location: 215 Hartwell
Time: 9:40 am (Session I)

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