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2012 Master's Level Graduate Research Conference

Session I - Session II - Session III - Session IV

Using Site-directed Mutagenesis to Identify the Most Immunogenic Regions of Protein Vaccine Candidate P6

Nontypable Haemophilus influenzae (NTHi) are gram-negative bacteria known to cause otitis media (ear infections), pneumonia, bronchitis, sinusitis and other invasive diseases. For over 25 years, the P6 protein has been a leading candidate for a protein vaccine against NTHi. The overall goal of our work is to identify the most immunogenic part of P6, since using that small fragment of P6 in a vaccine would be more cost effective than using the whole protein. We have used site-directed mutagenesis, recombinant protein expression, ELISA, and nuclear magnetic resonance spectroscopy to identify the residues on P6 which interact with monoclonal antibodies, since these residues are likely to be immunogenic.

Presenter: Anthony Mangan (Rochester Institute of Technology) --
Topic: Biology - Panel
Location: 122 Hartwell
Time: 10:30 am (Session II)