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Scholars Day 2006, Wednesday, April 12

Development of an Assay to Display Functional Motility in Zebrafish Larvae

Gastrointestinal (GI) motility is the coordinated contractions of smooth muscles resulting in mixing and propulsion of material through the GI tract. GI motility is influenced by smooth muscle, enteric neurons, and interstitial cells of Cajal (ICC). Regulation of GI motility is poorly understood, and the number of drugs which assist in GI motility disorders is insufficient. The overall goal of the laboratory is to establish a zebrafish based model system for GI motility. A vital part of this goal is the development of techniques that describe and quantify GI motility that leads to propulsion, versus GI motility that serves a mixing function. Contraction frequency of GI smooth muscle has been reported but it is unclear if these are mixing or propulsive contractions. Assays utilizing fluorescence microscopy and spectrophotometry were designed to measure propulsive motility, and were compared to digital video analysis that measures contraction frequency in wild type and mutant zebrafish.

Presenters: Stephanie Gross (Undergraduate Student)
Stacey Hess (Graduate Student)
Stacey Hess (Graduate Student)
Scott Leddon (Graduate Student)
Adam Rich (Faculty)
Topic: Biological Sciences
Location: 216 Hartwell
Time: 9:45 am (Session I)