Zebrafish, danio rerio, larvae are transparent, allowing for the direct visualization of internal organs while the organism remains intact. A histological atlas of the adult zebrafish GI tract is currently unavailable. Therefore, comparison at the tissue and cellular level with other model systems, and with human tissues, is difficult. Hematoxylin and Eosin (H&E) staining reveals cellular anatomy and enables identification of different cell types. H&E staining utilizes acid/base chemistry to enable easy viewing of the muscle layers, and the mucosal layers of the GI tract. The objective of this study is to produce a histological atlas of the adult zebrafish GI tract.. Adult zebrafish GI tissues were fixed in 4% paraformaldahyde, paraffin embedded, and sectioned in the transverse plane. H&E staining of tissue sections revealed a cellular organization similar to human and other mammalian model systems, with anterior and posterior GI tract corresponding to small and large intestine, respectively.
|Presenters:||Stephanie Gross (Undergraduate Student)
Stacey Hess (Graduate Student)
Scott Leddon (Graduate Student)
Kyle Leonard (Undergraduate Student)
Adam Rich (Faculty)
|Time:||10:15 am (Session I)|