Gastrointestinal (GI) motility is controlled by smooth muscles, enteric neurons, and interstitial cells of Cajal (ICC). ICC form a web-like network of cells between the longitudinal and circular layers of smooth muscle, ideally positioned to regulate motility. GI motility disorders are common but the underlying causes are not fully understood. Defects in ICC are associated with motility disorders in humans. Our goal is to develop a zebrafish-based model for GI motility. Coordinated motility is first evident in developing zebrafish larvae at 7 days post-fertilization (dpf). If ICC are necessary for coordinated GI contractions then ICC development will correlate with the development of GI motility. The specific objective of this project was to determine the stage when ICC first appear. Larvae were fixed, processed, embedded in paraffin wax, sectioned at 8ým, immunostained using anti-kit antibody, and digital images were recorded.
|Presenter:||Kyle Leonard (Undergraduate Student)|
|Time:||9:30 am (Session I)|