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Scholars Day 2007, Wednesday, April 11

Immunohistochemistry: The Basics

Pathology labs characterize tissue samples to guide physicians to diagnos and treat human disease. My objective was to master the techniques used to fix and section tissues. This presentation will cover the theoretical basis for tissue immunohistochemistry, and will present results from my project. An antibody that recognizes the proteins kita and kitb was used to determine the presence of interstitial cells of Cajal (ICC) in the larvae gastrointestinal tract. Briefly, larvae were fixed with 4% paraformaldehyde, processed to remove water and infused with paraffin wax, embedded in a wax block, and thinly sliced. These tissue sections were positioned on microscope slides. The kita and kitb protein are expressed on the ICC cell surface. Antibodies were used to specifically visualize protein expression. Our lab uses this technique to characterize ICC expression in the zebrafish larvae. Identical techniques are used clinically to characterize GI stromal tumors in humans.

Presenter: Stuart Ornduff (Undergraduate Student)
Topic: Biological Sciences
Location: 120 Hartwell
Time: 9:45 am (Session I)

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