The zebrafish is a popular model organism for development, and also for human disease. For example, the zebrafish cardiovascular system is similar to humans. The human GI tract mixes and propels the luminal contents with highly coordinated muscular contractions, and collectively the contractions are termed motility. The three main components for motility include Interstitial cells of Cajal (ICC), enteric neurons, and smooth muscles. The objective of my work is to determine the developmental time course for ICC in the zebrafish GI tract. Reverse transcriptase PCR will be used to determine the developmental stage that kita and kitb are expressed. Kit is a receptor tyrosine kinase in the signal transduction pathway that leads to ICC expression. Therefore, expression of Kit mRNA can be used to determine when ICC expression begins. These data will be correlated with protein expression and with functional GI motility measurements performed by other laboratory members.
|Presenter:||Sarah Blake (Graduate Student)|
|Time:||10 am (Session I)|