The enhancer of rudimentary gene, e(r )is a highly conserved gene located on the X chromosome in Drosophila melanogaster. The gene encodes a transcriptional co-factor 104 amino acids in length, and although the exact function is still unclear it has been shown to be involved in pyrimidine biosynthesis, oogenesis, and the cell cycle. Mutations in e(r) are characterized by low viability and decreased fertility. They have also been shown to enhance phenotypes caused by mutations in two genes involved in neurogenesis, Notch and deltex, suggesting a possible role in neurogenesis. In the present study, transgenes of e(r) are used to map the sequences necessary for normal expression. All of the sequences necessary for normal e(r) expression mapped to a 2,618-bp fragment that included the transcribed region plus 787 bp upstream of the start of transcription. The two introns of e(r) were shown not to contain transcription control sequences. These studies also mapped a transcription enhancer to a 5' region from -338 to -787 and a transcription silencer to a 5' region from -45 to -338.
|Presenter:||Nicholas Rizzo (Graduate Student)|
|Time:||1:15 pm (Session III)|