G protein-coupled receptors (GPCRs) are known to influence actin polymerization via several downstream signaling components; however, actin can also influence receptor trafficking, desensitization, and membrane stability. Little is known about how Melanin-concentrating hormone receptor (MCHR) 1 is influenced by actin, or how MCHR1 influences actin rearrangements. In Aim 1 of this study, the effect of MCH on 3T3-L1 pre-adipocyte actin morphology following MCH treatment was investigated for varying times. Slides were blinded and scored into three categories: 1) prominent actin stress fibers, 2) rounded cells with processes, and 3) small round cells. A statistically significant change in actin morphology was observed. In Aim 2, cultured cells stably expressing VSVg-tagged MCHR1 were pretreated with cytochalasin D to disrupt actin polymerization, treated with MCH, and ERK1/2 activation was measured. Cytochalasin D caused an increase in activated ERK1/2 expression. This data suggests actin may have a significant role in dampening receptor signaling.
|Presenter:||Scott Portwood (Graduate Student)|
|Time:||11:15 am (Session II)|