Melanin concentrating hormone (MCH) is a cyclic peptide which regulates a variety of physiological responses including color change in fish and feeding behavior in mammals. The receptor for MCH exists as two isoforms. Little is known about the regulation of MCH receptor-2, partly because of the lack of a high-affinity antibody typically used for protein assays. Using molecular cloning technology, a double hemagglutinin epitope tag was incorporated at the 5’ end of the cDNA coding sequence for human MCH receptor-2. The resulting plasmid was transfected into CHO cells and immunocytochemisty performed to assess localization to the plasma membrane. The development of a model cellular system for MCH receptor-2 will facilitate novel receptor trafficking and signaling studies. Preliminary Western blot experiments indicate that this receptor signals through ERK1/2, a previously unknown signaling pathway for this receptor and novel discovery.
|Presenter:||Bryon Tuthill (Graduate Student)|
|Time:||11:30 am (Session II)|