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Scholars Day: April 15, 2009

Structure and Phenotypic Analysis of Notch Mutations

The Notch gene encodes for a transmembrane protein that functions as a receptor for intracellular signals in Drosophila. The Notch gene is required for cell proliferation as well as differentiation. A Notch mutation, Nnd-p, is caused by the insertion of a P element into the first exon upstream of the start of translation Notch gene. The insertion causes a weak Notch mutation. New Notch mutations caused by the mobilization of the P element were isolated. Three of them (N2, N3, and N5) result in a further decrease in Notch activity. The fourth mutant is a revertant of the original Nnd-p mutation. Each mutation was analyzed by PCR and DNA sequencing to determine the precise structure of the mutation. The mutant phenotypes were studied by examining the viability of the larvae and adults.

Presenter: Melissa Carey (Graduate Student)
Topic: Biological Sciences
Location: 101 Edwards
Time: 11:20 am (Session II)