The Tsubota lab currently studies mutations in two Drosophila (fruit fly) genes, Notch (N) and enhancer of rudimentary (e(r)). Notch plays an important role in the differentiation of the exoskeleton and nervous system. E(r) appears to code a developmental transcription factor which acts on several genes including N and rudimentary. Both N and e(r) are located on the X chromosome. N8 is a deletion of the N allele, lethal during embryogenesis in males and homozygous females. Nnd-p is a p-element insertion in the 5’ utr which reduces the level of gene product. E(r)27-1 is a deletion which is lethal at the pupal stage when combined with Nnd-p in males or homozygous females. Inserting a green fluorescent protein gene in the wild type X chromosome allows rapid identification of males carrying the mutation during the larval stage and determination of the point in development at which lethality occurs.
|Presenter:||Phillip Bailey (Undergraduate Student)|
|Time:||11 am (Session II)|