Melanin-concentrating hormone (MCH) is an appetite-stimulating hormone, with receptors in the brain, pancreas and adipose. The broad distribution of its receptors suggests that MCH might help regulate the whole-body response to food. The aim of this study was to determine whether MCH alters migration in pre-adipocytes. 3T3-L1 pre-adipocytes, endogenously expressing MCH receptors, were wounded and then treated with MCH. At hour-long time points, the wound was measured and compared to the size of the initial wound. The closure was measured in both the presence and absence of MCH. MCH caused cells to migrate into the wound progressively faster. After six hours, the hormone-treated wounds had closed to 39.3±6.7% of the initial wound size versus the untreated wounds, which had only closed to 49.5±8.7% of the initial size. This novel finding suggests that while MCH does not influence pre-adipocyte differentiation, it could impact the overall development of adipose tissue in vivo.
|Presenter:||Laura Shum (Undergraduate Student)|
|Time:||9 am (Session I)|