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Scholars Day: April 7, 2010

Effects of MCH and PMC-3881-PI on the Activation of MAP Kinase Pathway

Effects of MCH and PMC-3881-PI on the Activation of MAP Kinase Pathway The melanin-concentrating hormone (MCH) is a hormone that regulates food intake and energy expenditure in mammals. When MCH binds to the receptor MCHR1, ERK1/2 is activated downstream in the MAP Kinase Pathway. The first goal of the experiment was to conduct a Bradford Assay to determine protein content in MCHR1. The second goal was to determine whether an MCH antagonist, PMC-3881-PI, could inhibit MCH mediated activation of the MAP Kinase Pathway. Western Blotting was used to find out if PMC-3881-PI blocked MCHR1 and ERK1/2 activation. The experiment showed that the antagonist blocked activity in the MAP Kinase Pathway.

Presenter: Stephanie Branigan (Undergraduate Student)
Topic: Biology
Location: 101 Edwards
Time: 9:15 am (Session I)

Events

Fri, Dec 5

Major Exploration Day
9 am - 3:15 pm