Effects of MCH and PMC-3881-PI on the Activation of MAP Kinase Pathway The melanin-concentrating hormone (MCH) is a hormone that regulates food intake and energy expenditure in mammals. When MCH binds to the receptor MCHR1, ERK1/2 is activated downstream in the MAP Kinase Pathway. The first goal of the experiment was to conduct a Bradford Assay to determine protein content in MCHR1. The second goal was to determine whether an MCH antagonist, PMC-3881-PI, could inhibit MCH mediated activation of the MAP Kinase Pathway. Western Blotting was used to find out if PMC-3881-PI blocked MCHR1 and ERK1/2 activation. The experiment showed that the antagonist blocked activity in the MAP Kinase Pathway.
|Presenter:||Stephanie Branigan (Undergraduate Student)|
|Time:||9:15 am (Session I)|