Melanin concentrating hormone (MCH) was first discovered in salmon where it acts to lighten skin color. In mammals, MCH has been shown to play a role in energy homeostasis through its two receptors melanin concentrating hormone receptors 1 & 2 (MCHR1 and MCHR2). MCHR1 has been shown to localize to the plasma membrane and partially internalize when exposed to MCH. However, little is known about MCHR2 since it is not natively expressed in rodents. Through cloning, four plasmids were created that contained either MCHR1 or MCHR2 cDNA tagged with HA or Myc. BHK570 cells were transfected with these plasmids and fluorescence microscopy and immunocytochemistry were used to test for and compare receptor localization at the plasma membrane as well as receptor internalization when exposed to MCH.
|Presenter:||Jay Moden (Graduate Student)|
|Time:||9:45 am (Session I)|