Melanin-concentrating hormone receptor 1 (MCHR1) knockout mice have limited incidence of diet-induced obesity. This makes it a possible drug target to fight human obesity. G-protein coupled receptors which are often internalized to prevent overstimulation of the cell. Our lab has previously determined that less than 15% of MCHR1 internalizes within 30 min of MCH treatment, despite rapid and extensive ERK desensitization. Aim 1 was to determine if long-term MCH treatment produced internalization of receptors on a larger scale. We have been unable to detect significant changes in receptor distribution by microscopy. Contrary to our expectations, Western results indicate there may be more MCHR1 with overnight MCH treatment. Aim 2 was to further confirm ERK desensitization by MCH and quantify the time course of signal recovery. We were able to confirm early ERK experiments demonstrating desensitization by MCH and are now moving studies into a more relevant cell model.
|Presenter:||Andrew Goodspeed (Undergraduate Student)|
|Time:||9 am Session I|