Distinguishing different smells depends on the organization of olfactory sensory inputs in the olfactory bulb or olfactory map. Understanding how the map is built during embryogenesis is critical to understand how our sense of smell develops. In Drosophila antennal lobe, the olfactory receptor axons target the dendrites of projection neuron to form a three-dimensional synaptic map. We found that the wnt5 gene is needed for the precise patterning of this map. We propose the Wnt5 protein is expressed by guidepost? cells in restricted domains of developing antennal lobe, and its loss of expression severely disrupts the patterning of the dendritic arbors. To characterize these guidepost cells, we have performed the process of placing the Gal4 gene under the control of the wnt5 promoter using homologous recombination. We have screened 306,200 animals where 320 were found to have Gal4 mobilization and 18 have been identified to be X-linked.
|Presenter:||Jay-Christian Helt (Graduate Student)|
|Time:||11 am Session II|