Gastrointestinal (GI) motility mixes, and propels intestinal contents in an anterograde direction and is necessary for nutrient absorption and elimination of waste. Interstitial cells of Cajal (ICC), enteric neurons, and smooth muscle cells all regulate GI motility. The Kit receptor is expressed on ICC and stimulation by kit ligand is necessary for ICC development in mouse and humans. The zebrafish expresses 2 Kit orthologs, kita and kitb, and 2 Kit Ligand orthologs, kitla and kitlb. ICC growth and development is poorly understood in humans, and the relationship between the 2 zebrafish kit ligands and kit receptors has not been determined. The objective of these experiments was to verify that the sequence of the kita gene found in the zebrafish database was correct. The kitA gene was amplified by PCR from zebrafish cDNA and, if the size of the PCR product correct, cloned into the plasmid pCR-TOPO. The strategy and progress of this cloning project will be discussed.
|Presenter:||Isabelle Blair (Undergraduate Student)|
|Time:||3 pm Session IV|