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Scholars Day: April 11, 2012

Cloning of the KitA Gene From Zebrafish

Gastrointestinal (GI) motility mixes, and propels intestinal contents in an anterograde direction and is necessary for nutrient absorption and elimination of waste. Interstitial cells of Cajal (ICC), enteric neurons, and smooth muscle cells all regulate GI motility. The Kit receptor is expressed on ICC and stimulation by kit ligand is necessary for ICC development in mouse and humans. The zebrafish expresses 2 Kit orthologs, kita and kitb, and 2 Kit Ligand orthologs, kitla and kitlb. ICC growth and development is poorly understood in humans, and the relationship between the 2 zebrafish kit ligands and kit receptors has not been determined. The objective of these experiments was to verify that the sequence of the kita gene found in the zebrafish database was correct. The kitA gene was amplified by PCR from zebrafish cDNA and, if the size of the PCR product correct, cloned into the plasmid pCR-TOPO. The strategy and progress of this cloning project will be discussed.

Please note that presentation times are approximate. If you are interested in attending sessions with multiple presentations, please be in the room at the start of the session.
Presenter: Isabelle Blair (Undergraduate Student)
Topic: Biology
Location: 217 Hartwell
Time: 3 pm Session IV