Simple Green is a cleaning product used primarily in the household and claims to be environmentally safe. 2-Butoxyethanol is the only listed chemical in Simple Green with an established exposure limit. Chinese hamster ovary cells of the K-1 strain (CHO-K1) in multi-well plates were exposed to 0%, 0.1%, 1.0% or 10% Simple Green for 10 minutes, and were kept at a temperature of 37°C and a pH of 7.2. Cell viability followed a linear path as the concentration of Simple Green was increased. We observed: 2.4% viability at [10%], 49.5% at [1.0%], and 77.1% at [0.1%] when compared to the control culture. According to the data received when plated on mammalian tissue cells, even a 1:100 dilution of Simple Green caused 97.6% of cells to die when compared to control treatments. Sunshine Makers Inc.'s claim to be environmentally safe is not supported by the data observed and this raises the question of - What effect does this "environmentally safe" product have on other cells and microorganisms?
|Presenters:||Casey Donovan (Undergraduate Student)
Andrew Goodspeed (Undergraduate Student)
Matthew Morasco (Undergraduate Student)
Austin Olek (Undergraduate Student)
|Time:||11:15 am Session II|