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Scholars Day: April 11, 2012

CHO-K1 and BHK Cell Exposure to 'Environmentally Safe' Simple Green and 2-Butoxyethanol Has an Alarming Effect In Cell Viability

Simple Green is a cleaning product used primarily in the household and claims to be environmentally safe. 2-Butoxyethanol is the only listed chemical in Simple Green with an established exposure limit. Chinese hamster ovary cells of the K-1 strain (CHO-K1) in multi-well plates were exposed to 0%, 0.1%, 1.0% or 10% Simple Green for 10 minutes, and were kept at a temperature of 37C and a pH of 7.2. Cell viability followed a linear path as the concentration of Simple Green was increased. We observed: 2.4% viability at [10%], 49.5% at [1.0%], and 77.1% at [0.1%] when compared to the control culture. According to the data received when plated on mammalian tissue cells, even a 1:100 dilution of Simple Green caused 97.6% of cells to die when compared to control treatments. Sunshine Makers Inc.'s claim to be environmentally safe is not supported by the data observed and this raises the question of - What effect does this "environmentally safe" product have on other cells and microorganisms?

Please note that presentation times are approximate. If you are interested in attending sessions with multiple presentations, please be in the room at the start of the session.
Presenters: Casey Donovan (Undergraduate Student)
Andrew Goodspeed (Undergraduate Student)
Matthew Morasco (Undergraduate Student)
Austin Olek (Undergraduate Student)
Topic: Biology
Location: 217 Hartwell
Time: 11:15 am Session II