Introduction: Anoctamin 1 (Ano1) is a calcium activated chloride selective channel that was recently discovered as a marker for gastrointestinal stromal tumors and is expressed on interstitial cells of Cajal (ICC). The functional role of Ano1 on ICC is unknown. Objective: Determine protein expression of Ano1 in the developing zebrafish GI tract. Methods: Ano1 expression will be determined using anti-Ano1 antibodies and fluorescence microscopy. Discussion: ICC are necessary for development and maintenance of coordinated GI motility patterns. Ano1 is expressed in human ICC and may be important for pacemaker activity. The zebrafish model will be used to test the hypothesis that Ano1 contributes to spontaneous rhythmic contractions. Protocols for Ano1 immunohistochemistry in developing zebrafish will be established by these experiments.
|Presenter:||Julie McGrath (Undergraduate Student)|
|Time:||9:15 am (Session I)
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