Introduction: Piezo is a newly discovered ion channel that responds to changes in membrane tension by opening. Piezo is expressed in sensory neurons, consistent with a role in touch and pain sensation and partially regulates epithelial cell turnover in response to changes in membrane tension induced by cell crowding. The gastrointestinal tract is sensitive to stretch and Piezo may be involved in this process. Objective: Determine expression patterns of Piezo in the zebrafish gastrointestinal tract. Methods: Piezo expression in zebrafish was examined using reverse transcriptase PCR and RNA isolated from adult gastrointestinal tissue and intact 6 day old larvae. Results: Piezo expression was observed in adult zebrafish GI tissues, and in 6 day old zebrafish. Summary: Expression of Piezo was confirmed in zebrafish GI tissues and in larvae. Future experiments will determine the Piezo spatial expression pattern. The physiological role for Piezo in zebrafish will be determined using GsMTx4 toxin.
|Presenter:||Alexander Viavattine (Undergraduate Student)|
|Time:||9 am (Session I)
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