Introduction: Our laboratory uses the zebrafish model for human gastrointestinal motility. The current focus is to determine the physiological and developmental roles for anoctamin 1 and anoctamin 2 (Ano1 and Ano2). In collaboration with Jeffery Amack’s lab at SUNY Upstate Medical University we are developing transgenic models lacking Ano1 or Ano2. The role of our lab is to perform physiologic assays on these models to determine effects on zebrafish GI motility, and on cilia function during development. Methods: Ano1 or Ano2 expression in developing embryos will be knocked down using morpholino oligonucleotides. Knockdown will be verified using molecular techniques. Digital imaging of intact, living zebrafish larvae will be used to measure GI motility and cilia function at appropriate developmental time points. . . Discussion: This is an ongoing collaborative project. This talk will describe our approach, progress, and future plans. The perspective of a new unexperienced research student will be presented.
|Presenter:||Clayton Brady (Undergraduate Student)|
|Time:||9:50 am Session I|