Saccharomyces cerevisiae has long been studied as a eukaryotic model cell. Mitochondria are essential organelles found in all eukaryotes. Its proper function relies on both nuclear and mitochondrial gene products. S. cerevisiae is a facultative anaerobe that can thrive without respiration. However, appropriate mitochondrial function is necessary so that these cells remain viable. A yeast two-hybrid assay with the known mitochondrial protein, Ilv5p, was used to isolate other genes involved in stabilizing the mitochondrial genome. One of the genes identified was FMP35. FMP35 is a novel gene found on chromosome IX of S. cerevisiae. It is a previously identified protein localized to the mitochondria. A deletion of this gene was not lethal to the cell but resulted in the loss of normal mitochondrial function as assayed by an increased loss of respiration phenotype. To further characterize the mechanism leading to the loss of normal mitochondrial function, genetic assays were used. Loss of Fmp35p function displayed an increase in microsatellite instability and recombination rate that could lead to the loss of normal mitochondrial function.
|Presenter:||Chad Cornelius (Graduate Student)|
|Time:||1:45 pm (Session III)|